2014, Volume 6, Issue 4
Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?
Zbigniew Jastrzebski1, Malgorzata Zychowska1
1Department of Tourism and Recreation, Gdansk University of Physical Education and Sport in Gdansk
Author for correspondence: Zbigniew Jastrzebski; Department of Tourism and Recreation, Gdansk University of Physical Education and Sport in Gdansk; email: zb.jastrzebski[at]op.pl
Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max).
Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the
ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 µg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ∆∆Ct method was used.
Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion.
Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated.
Key words: gene expression, rowers, physical performance, leukocytes