2016, Volume 8, Issue 1
Changes in leukocyte HSPA1A, HSPB1 mRNA in basketball players after plyometric training
Małgorzata Żychowska1, Eglė Kemerytė-Riaubienė2, Audrius Gocentas3, Nijole Jascaniniene4, Grzegorz Chruściński5
1Department of Life Sciences, Gdansk University of Physical Education and Sport
2Department Department of Theory of Sport, Lithuanian University of Educational Sciences
3Department of Health and Physical Education, Lithuanian University of Educational Sciences
4Department of Theory of Sport, Lithuanian University of Educational Sciences
5Department of Sports Management, Gdansk University of Physical Education and Sport
Author for correspondence: Małgorzata Żychowska; Department of Life Sciences, Gdansk University of Physical Education and Sport; email: email@example.com
Background: Exercise-induced stressors activate leukocyte HSPA1A and HSPB1 gene transcripts. However it is not clear how plyometric training affects the expression of these genes in basketball players under plyometric exercise. Therefore, the aim of this study was to investigate the changes in leukocyte HSPA1A and HSPB1 mRNA, in male basketball players after plyometric training.
Material/Methods: Twelve male college basketball players (age 22.1 ±2.96 years) took part in this study. Peripheral blood (2.0 ml) was collected from the ulnar vein of each participant before and after a plyometric exercise to assess HSPA1A and HSPB1 mRNA relative expression of leukocyte via quantitative reverse transcription polymerase chain reaction.
Results: A significant increment of leukocyte HSPA1A mRNA expression (Qt from 1.67 ±0.93 to 3.17 ±0.97, p = 0.003) after plyometric exercise was found. However, there was no significant change in leukocyte HSPB1 mRNA expression, indicating the high stability of this gene during exercises.
Conclusions: HSPA1A mRNA was found to be a very sensitive indicator and could be used to assess physiological adaptation to a physical load and time requirements for complete recovery in basketball players.
Key words: gene expression, heat shock proteins, basketball, plyometric training